What is the Huh7 cell line?

Huh-7 is an immortal cell line composed of epithelial-like, tumorigenic cells. The majority of Huh-7 cells show a chromosome number between 55 and 63 (mode 60) and are highly heterogeneous. These cells are adherent to the surface of flasks or plates and typically grow as 2D monolayers. Although containing many mutations and INDELS, it is worth to note the Huh7 cells have a point mutation in the p53 gene.

Huh7 cell line origin:

The HuH-7 cell line was established in 1982 from a well-differentiated hepatocyte-derived cellular carcinoma cell line that was originally taken from a liver tumor in a 57-year-old Japanese male. The line was established by Nakabayshi, H., and Sato, J. Huh-7 is highly susceptible to the hepatitis C virus (HCV), and it is often used as a model to investigate hepatoma and HCV. This cell line can be used for an HCV replicon system, which allows for an in vitro production of infectious HCV particles and the development of drugs that combat HCV.